PDGFRA Monoclonal antibody

PDGFRA Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2C11B8

Cat no : 60045-1-Ig

Synonyms

CD140A, CD140a antigen, PDGF R alpha, PDGF receptor alpha, PDGFR2, PDGFRA, PDGFRα, PDGFR-α, Rhe PDGFRA



Tested Applications

Positive WB detected inTHP-1 cells, HT-1080 cells, HUVEC cells, U-251 cells
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

60045-1-Ig targets PDGFRA in WB, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDGFRA fusion protein Ag1385
Full Name platelet-derived growth factor receptor, alpha polypeptide
Calculated molecular weight 123 kDa
Observed molecular weight 100-120 kDa, 140-160 kDa
GenBank accession numberBC015186
Gene symbol PDGFRA
Gene ID (NCBI) 5156
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival and chemotaxis. Mutations in PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Protocols

Product Specific Protocols
WB protocol for PDGFRA antibody 60045-1-IgDownload protocol
FC protocol for PDGFRA antibody 60045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Metabolism

The vitamin D receptor regulates miR-140-5p and targets the MAPK pathway in bone development.

Authors - Wenting Luo
mouseIF

Development

The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.

Authors - Tiffany L Dill