Tested Applications
| Positive WB detected in | A431 cells, human placenta tissue, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17134-1-AP targets PDHA2/PDHA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10882 Product name: Recombinant human PDHA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-388 aa of BC030697 Sequence: MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLLEEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGLEAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS Predict reactive species |
| Full Name | pyruvate dehydrogenase (lipoamide) alpha 2 |
| Calculated Molecular Weight | 388 aa, 43 kDa |
| Observed Molecular Weight | 40-43 kDa |
| GenBank Accession Number | BC030697 |
| Gene Symbol | PDHA2 |
| Gene ID (NCBI) | 5161 |
| RRID | AB_2878352 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29803 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDHA2 (Pyruvate Dehydrogenase E1 Subunit Alpha 2) is a testis-specific mitochondrial enzyme encoded by the PDHA2 gene on human chromosome 4q22.3. The gene exhibits 84% nucleotide sequence similarity with the X-linked PDHA1, supporting the hypothesis that PDHA2 originated through retrotransposition of PDHA1 to an autosomal location. This antibody can recognize both PDHA2 and PDHA1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDHA2/PDHA1 antibody 17134-1-AP | Download protocol |
| WB protocol for PDHA2/PDHA1 antibody 17134-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDHA2/PDHA1 Polyclonal antibody (Cat# 17134-1-AP) has 3 total citations. These appear in Metabolism (hepatic metabolic crisis and mitochondrial oxidative stress), Cell Death & Disease (sperm flagellum formation and male fertility in mice), and MethodsX (purification of murine cortex mitochondria). The cited research fields span mitochondrial biology, reproductive biology, and cell isolation methods, with WB as the cited application in mouse models.
| Species | Application | Title |
|---|---|---|
Metabolism Glutamine synthetase deficiency triggers mitochondrial oxidative stress and a ribotoxic stress response during acute hepatic metabolic crisis. | ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice | ||
MethodsX Purification of murine cortex mitochondria using a modified magnetic bead isolation method. |
