Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, human heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14744-1-AP targets PDHB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6442 Product name: Recombinant human PDHB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-359 aa of BC000439 Sequence: MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI Predict reactive species |
| Full Name | pyruvate dehydrogenase (lipoamide) beta |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC000439 |
| Gene Symbol | PDHB |
| Gene ID (NCBI) | 5162 |
| RRID | AB_2162941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11177 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDHB(Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named as PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDHB antibody 14744-1-AP | Download protocol |
| IHC protocol for PDHB antibody 14744-1-AP | Download protocol |
| IP protocol for PDHB antibody 14744-1-AP | Download protocol |
| WB protocol for PDHB antibody 14744-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PDHB antibody (Cat# 14744-1-AP) has 39 total citations across journals including Nature Communications, Cell Research, Molecular Cell, Science Advances, Circulation Research, BMC Cancer, Oncotarget, and Phytomedicine. Research fields span cancer metabolism (gastric, ovarian, renal, breast, cervical), cuproptosis, mitochondrial pyruvate metabolism, neurodegeneration (Parkinson's, Alzheimer's), necroptosis/inflammation, liver disease, osteonecrosis, and sepsis. Applications are predominantly WB, with some IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Imeta Clinical features and molecular landscape of cuproptosis signature-related molecular subtype in gastric cancer | ||
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis | ||
Nat Commun The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression. | ||
Nat Commun TGFβ-activated PDHB promotes mitochondrial pyruvate metabolism and contributes to human endoderm differentiation via ATP-dependent BRG1.
| ||
Nat Commun A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism |
