PDHB Monoclonal antibody, PBS Only

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  WB analysis using 68238-1-Ig (same clone as 68238-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68238-1-Ig (same clone as 68238-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

PDHB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68238-1-PBS

Clone No.2A7C10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2A7C10, EC:1.2.4.1, PDHE1B, PDHE1-B, PHE1B

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Product Information

68238-1-PBS targets PDHB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6857

  Product name: Recombinant human PDHB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-359 aa of BC000439

  Sequence: MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI

• Full Name: pyruvate dehydrogenase (lipoamide) beta
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000439
• Gene Symbol: PDHB
• Gene ID (NCBI): 5162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P11177
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).