ERp57/ERp60 Monoclonal antibody

ERp57/ERp60 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G4G7

Cat no : 66423-1-Ig

Synonyms

ERp57, PDIA3, ER60, EC:5.3.4.1, Disulfide isomerase ER-60



Tested Applications

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, LNCaP cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66423-1-Ig targets ERp57/ERp60 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERp57/ERp60 fusion protein Ag8741
Full Name protein disulfide isomerase family A, member 3
Calculated Molecular Weight 505 aa, 57 kDa
Observed Molecular Weight 57 kDa, 60 kDa
GenBank Accession NumberBC014433
Gene Symbol PDIA3
Gene ID (NCBI) 2923
RRIDAB_2881795
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Protocols

Product Specific Protocols
WB protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
IHC protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
IF protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols