PDIA4 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86239-1-RR (same clone as 86239-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86239-1-RR (PDIA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 86239-1-RR (same clone as 86239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDIA4 antibody (86239-1-RR, Clone: 250697E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

PDIA4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86239-1-PBS

Clone No.250697E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ERp72, EC:5.3.4.1, ERp 72, ERp70, ERp-72

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Product Information

86239-1-PBS targets PDIA4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3011

  Product name: Recombinant Human PDIA4/ERp72 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-641 aa of NM_004911.5

  Sequence: VAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRT

• Full Name: protein disulfide isomerase family A, member 4
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_004911.5
• Gene Symbol: ERp72
• Gene ID (NCBI): 9601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13667
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.