PDIR Recombinant antibody

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  WB analysis using 85599-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85599-4-RR (PDIA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85599-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85599-4-RR against Human PDIR were performed. The affinity constant is below 1 pM.

View 3D epitope

PDIR Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85599-4-RR

Clone No.242971D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, HeLa cells, MCF-7 cells, Jurkat cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85599-4-RR targets PDIR in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDIR fusion protein Ag31479
• Full Name: protein disulfide isomerase family A, member 5
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC001625
• Gene Symbol: PDIR
• Gene ID (NCBI): 10954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14554
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIR(Protein disulfide isomerase-related protein), also known as PDIA5 (Protein Disulfide Isomerase Family A Member 5), is a member of the PDI gene family and also exhibits chaperone-like activity. PDIR protein is correlated with immune infiltration, which is highly expressed in glioma and participates in glioma progression(PMID: 33664747). PDIR contributes to disulfide bond rearrangement in ATF6α under stress conditions that necessary for ATF6α activation upon ER stress(PMID: 24636989).