PDK1 Polyclonal antibody
PDK1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IP, IHC, ELISA
Cat no : 10026-1-AP
Validation Data Gallery
|Positive WB detected in||mouse liver tissue, mouse small intestine tissue, NIH/3T3 cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human small intestine tissue, human colon tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10026-1-AP targets PDK1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PDK1 fusion protein Ag0025|
|Full Name||pyruvate dehydrogenase kinase, isozyme 1|
|Calculated molecular weight||436 aa, 49 kDa|
|Observed molecular weight||46-55 kDa|
|GenBank accession number||BC039158|
|Gene ID (NCBI)||5163|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is speicfic to PDK1.
|Product Specific Protocols|
|WB protocol for PDK1 antibody 10026-1-AP||Download protocol|
|IHC protocol for PDK1 antibody 10026-1-AP||Download protocol|
|IP protocol for PDK1 antibody 10026-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.
Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma
J Cell Mol Med
INPP4B restrains cell proliferation and metastasis via regulation of the PI3K/AKT/SGK pathway.
The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer.
Biochim Biophys Acta Gen Subj
Novel function of Roxadustat (FG-4592) as an anti-shock drug in sepsis by regulating mitochondrial oxidative stress and energy metabolism
Deoxyelephantopin decreases the release of inflammatory cytokines in macrophage associated with attenuation of aerobic glycolysis via modulation of PKM2.