PDK1 Polyclonal antibody

PDK1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10026-1-AP

Synonyms

PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1



Tested Applications

Positive WB detected inmouse liver tissue, mouse small intestine tissue, NIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman small intestine tissue, human colon tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10026-1-AP targets PDK1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDK1 fusion protein Ag0025
Full Name pyruvate dehydrogenase kinase, isozyme 1
Calculated molecular weight 436 aa, 49 kDa
Observed molecular weight 46-55 kDa
GenBank accession numberBC039158
Gene symbol PDK1
Gene ID (NCBI) 5163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is speicfic to PDK1.

Protocols

Product Specific Protocols
WB protocol for PDK1 antibody 10026-1-APDownload protocol
IHC protocol for PDK1 antibody 10026-1-APDownload protocol
IP protocol for PDK1 antibody 10026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.

Authors - Lina Zhou
humanWB

FASEB J

Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.

Authors - Erhui Jiang
humanIHC

EBioMedicine

Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer.

Authors - Wei Peng
humanWB

Proc Natl Acad Sci U S A

LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.

Authors - Shaoxun Xiang
humanWB

Biosci Rep

Loss of protein targeting to glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.

Authors - Rongqiang Yang
ratWB

J Cell Mol Med

PMID: 31945804

Authors - Chenyang Chen