PDK4 Polyclonal antibody, PBS Only

Name: PDK4 Polyclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 12949-1-PBS-100UG

PDK4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12949-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, EC:2.7.11.2, PDHK4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIHC

WB analysis using 12949-1-AP (same clone as 12949-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
WB analysis using 12949-1-AP (same clone as 12949-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12949-1-PBS targets PDK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3629 Product name: Recombinant human PDK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 61-412 aa of BC040239 Sequence: LPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM Predict reactive species
Full Name	pyruvate dehydrogenase kinase, isozyme 4
Calculated Molecular Weight	411 aa, 46 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC040239
Gene Symbol	PDK4
Gene ID (NCBI)	5166
RRID	AB_2161499
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16654
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).