PDLIM2 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• PDLIM2 Polyclonal antibody (11574-1-AP)

PDLIM2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11574-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MYSTIQUE, PDZ and LIM domain protein 2, PDZ LIM protein mystique, PDZ-LIM protein mystique, PP6345

• 

  WB analysis using 11574-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11574-1-AP (PDLIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11574-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11574-1-AP (PDLIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: DU 145 cells, Reh cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11574-1-AP targets PDLIM2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2155

  Product name: Recombinant human PDLIM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-352 aa of BC021556

  Sequence: MALTVDVAGPAPWGFRITGGRDFHTPIMVTKVAERGKAKDADLRPGDIIVAINGESAEGMLHAEAQSKIRQSPSPLRLQLDRSQATSPGQTNGDSSLEVLATRFQGSVRTYTESQSSLRSSYSSPTSLSPRAGSPFSPPPSSSSLTGEAAISRSFQSLACSPGLPAADRLSYSGRPGSRQAGLGRAGDSAVLVLPPSPGPRSSRPSMDSEGGSLLLDEDSEVFKMLQENREGRAAPRQSSSFRLLQEALEAEERGGTPAFLPSSLSPQSSLPASRALATPPKLHTCEKCSTSIANQAVRIQEGRYRHPGCYTCADCGLNLKMRGHFWVGDELYCEKHARQRYSAPATLSSRA

• Full Name: PDZ and LIM domain 2 (mystique)
• Calculated Molecular Weight: 37.5 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC021556
• Gene Symbol: PDLIM2
• Gene ID (NCBI): 64236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96JY6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PDZ and LIM domain-containing protein 2(PDLIM2), also known as Mystique or SLIM, is classified as a member of the actinin-associated LIM family of proteins. PDLIM2 is an IGF-IR-regulated adapter protein located at the actin cytoskeleton and necessary for the migratory capacity of epithelial cells (PMID: 15659642, 34571051). PDLIM2 is a nuclear ubiquitin E3 ligase that negatively regulates STAT signaling(PMID: 15963787).