PDLIM3 Polyclonal antibody

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• PDLIM3 Polyclonal antibody (13199-2-AP)

PDLIM3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13199-2-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ALP, Actinin associated LIM protein, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein, PDZ and LIM domain 3

• 

  WB analysis of human heart using 13199-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 13199-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 13199-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13199-2-AP (PDLIM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human heart tissue, mouse spleen tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13199-2-AP targets PDLIM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3934

  Product name: Recombinant human PDLIM3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC027870

  Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQNEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA

• Full Name: PDZ and LIM domain 3
• Calculated Molecular Weight: 316 aa, 34 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC027870
• Gene Symbol: PDLIM3
• Gene ID (NCBI): 27295
• RRID: AB_2161653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53GG5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM3, also known as ALP, contains a PDZ domain and a LIM domain. PDLIM3 binds the spectrin-like repeats of alpha-actinin-2 and colocalizes with alpha-actinin-2 at the Z lines of skeletal muscle.

Publications

What published studies show

Anti-PDLIM3 antibody (Cat# 13199-2-AP) has 5 total citations across journals including Gut Microbes, Small, J Tissue Eng, Int J Mol Sci, and Cell Mol Biol. Research fields span gut dysbiosis and colitis, osteoporotic bone regeneration via mitophagy, vascularization and tissue engineering, head and neck squamous cell carcinoma (EMT), and osteoblast proliferation/differentiation. Most studies applied the antibody in Western blot with mouse samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Gut Microbes; Ultra-processed foods sourced 7-ketositosterol aggravates colitis through gut dysbiosis induced-PDLIM3 activation.; Authors - Jing Yan
mouse	WB	Small; Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy; Authors - Dan Li
mouse	WB	J Tissue Eng; Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration; Authors - Shijie Fan
		Int J Mol Sci; PDLIM3 Regulates Migration and Invasion of Head and Neck Squamous Cell Carcinoma via YAP-Mediated Epithelial-Mesenchymal Transition; Authors - Fan Yang
		Cell Mol Biol (Noisy-le-grand); Isofraxidin inhibits the proliferation and differentiation of the osteoblast MC3T3-E1 subclone 14 cell line; Authors - Junliang Xu