PDLIM4 Polyclonal antibody, PBS Only

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• PDLIM4 Polyclonal antibody, PBS Only (18543-1-PBS)

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  WB analysis using 18543-1-AP (same clone as 18543-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18543-1-AP (PDLIM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 18543-1-AP (same clone as 18543-1-PBS)

  WB result of PDLIM4 antibody (18543-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM4 transfected A431 cells. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 18543-1-AP (same clone as 18543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18543-1-AP (PDLIM4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 18543-1-AP (same clone as 18543-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PDLIM4 antibody (18543-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PDLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18543-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PDZ and LIM domain 4, Reversion-induced LIM protein, RIL

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Product Information

18543-1-PBS targets PDLIM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13424

  Product name: Recombinant human PDLIM4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC016765

  Sequence: MPHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPEIQDGSPTTSRGPSGTGTGPEDGRPSLGSPYGQPPRFPVPHNGSSEATLPAQMSTLHVSPPPSADPARGLPRSRDCRVDLGSEVYRMLREPAEPVAAEPKQSGSFRYLQGMLEAGEGGDWPGPGGPRNLKPTASKLGAPLSGLQGLPECTRCGHGIVGTIVKARDKLYHPECFMCSDCGLNLKQRGYFFLDERLYCESHAKARVKPPEGYDVVAVYPNAKVELV

• Full Name: PDZ and LIM domain 4
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC016765
• Gene Symbol: PDLIM4
• Gene ID (NCBI): 8572
• RRID: AB_3085565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50479
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).