PDLIM5 Polyclonal antibody

• Featured Product
• KD/KO Validated

PDLIM5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10530-1-AP

Synonyms

PDZ and LIM domain 5, L9, Enigma-like PDZ and LIM domains protein, ENH

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Validation Data Gallery

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  WB analysis of HeLa using 10530-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10530-1-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 antibody) at dilution of 1:250.

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  WB analysis of HUVEC using 10530-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10530-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10530-1-AP

  IP result of anti-PDLIM5 (IP:10530-1-AP, 4ug; Detection:10530-1-AP 1:300) with A549 cells lysate 2400ug.

• 

  IHC staining of human stomach cancer using 10530-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10530-1-AP (PDLIM5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HUVEC cells, NIH/3T3 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

10530-1-AP targets PDLIM5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDLIM5 fusion protein Ag0808
• Full Name: PDZ and LIM domain 5
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 63-68 kDa
• GenBank Accession Number: BC008741
• Gene Symbol: PDLIM5
• Gene ID (NCBI): 10611
• RRID: AB_2161779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression. This antibody is specific to PDLIM5, and its specificity has been confirmed by siRNA test.

Publications

Species	Application	Title
mouse	WB	Cell Death Differ; MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis.; Authors - H Qiu ; KD Validated
human	WB,IHC	Oncotarget; High expression of PDLIM5 facilitates cell tumorigenesis and migration by maintaining AMPK activation in prostate cancer.; Authors - Xi Liu ; KD Validated
human	WB	Drug Deliv; Ultrasound-sensitive siRNA-loaded nanobubbles fabrication and antagonism in drug resistance for NSCLC.; Authors - Chunhong Su
rat	WB	J Mol Cell Cardiol; Extracellular vesicular MicroRNA-27a* contributes to cardiac hypertrophy in chronic heart failure.; Authors - Changhai Tian ; KD Validated
human	WB	Chem Biol Drug Des; si-RNA mediated knockdown of PDLIM5 suppresses gastric cancer cell proliferation in vitro.; Authors - Yanliang Li ; KD Validated
mouse	IHC	Sci Rep; Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration; Authors - Kento Takaya