|Positive WB detected in||SKOV-3 cells, HeLa cells, MCF-7 cells|
|Positive IP detected in||HeLa cells, MCF-7 cells|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SKOV-3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and N/A for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10221-1-AP targets PDLIM7 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PDLIM7 fusion protein Ag0264|
|Full Name||PDZ and LIM domain 7 (enigma)|
|Calculated molecular weight||55/60 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC001093|
|Gene ID (NCBI)||9260|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.
|Product Specific Protocols|
|WB protocol for PDLIM7 antibody 10221-1-AP||Download protocol|
|IHC protocol for PDLIM7 antibody 10221-1-AP||Download protocol|
|IF protocol for PDLIM7 antibody 10221-1-AP||Download protocol|
|IP protocol for PDLIM7 antibody 10221-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity.
J Mol Histol
Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development.
Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation.
Int J Mol Sci
LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells.
J Orthop Res
LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.
Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This antibody worked quite well in detecting PDLIM7 in mouse heart protein lysate.
Joleen (Verified Customer) (06-10-2019)
The most prominent band correspond to where PDLIM7 is expected to be. But there are other surrounding background bands.