Tested Applications
| Positive WB detected in | SKOV-3 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human colon tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10221-1-AP targets PDLIM7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0264 Product name: Recombinant human PDLIM7,LMP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-289 aa of BC001093 Sequence: SFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVI Predict reactive species |
| Full Name | PDZ and LIM domain 7 (enigma) |
| Calculated Molecular Weight | 55/60 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC001093 |
| Gene Symbol | PDLIM7 |
| Gene ID (NCBI) | 9260 |
| RRID | AB_2161789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
| IHC protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
| IP protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
| WB protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDLIM7 Polyclonal antibody (Cat# 10221-1-AP) has 16 citations published in journals including Signal Transduction and Targeted Therapy, Advanced Science, Cell Reports, EMBO Reports, Structure, Journal of Bone and Mineral Research, Frontiers in Immunology, Cell Biosciences, International Journal of Molecular Sciences, and Scientific Reports. Research fields span adipogenesis and obesity, breast and prostate cancer, osteogenesis, vascular calcification, inflammatory signaling, dental development, intervertebral disc degeneration, and mesenchymal stem cell biology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Annexin A1 binds PDZ and LIM domain 7 to inhibit adipogenesis and prevent obesity | ||
Adv Sci (Weinh) Osmotic Tension Asymmetry Drives Electrotactic Migration via PDLIM7-Polarized Microfilament Coordination in Breast Cancer Cells.
| ||
Cell Rep Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity. | ||
Front Immunol PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB. | ||
Cell Biosci TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1 | ||
J Bone Miner Res LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2 Mediated Osteogenesis through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity. |
Reviews
What customers say
Users report that this antibody performs well in Western Blot for detecting PDLIM7. One reviewer confirmed clear detection in mouse heart protein lysate, while another observed the expected band in MDCK GII cells at a 1:1,000 dilution, though some surrounding background bands were noted. Overall, the antibody is considered effective for PDLIM7 detection, with minor background as the main drawback.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (08-25-2020) | This antibody worked quite well in detecting PDLIM7 in mouse heart protein lysate.
|
Joleen (Verified Customer) (06-10-2019) | The most prominent band correspond to where PDLIM7 is expected to be. But there are other surrounding background bands.
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