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  • KD/KO Validated

PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10221-1-AP

Synonyms

ENIGMA, LIM mineralization protein, LMP, PDLIM7, PDZ and LIM domain 7 (enigma), PDZ and LIM domain protein 7, Protein enigma



Tested Applications

Positive WB detected inSKOV-3 cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and N/A for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10221-1-AP targets PDLIM7 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDLIM7 fusion protein Ag0264
Full Name PDZ and LIM domain 7 (enigma)
Calculated molecular weight 55/60 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001093
Gene symbol PDLIM7
Gene ID (NCBI) 9260
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

Protocols

Product Specific Protocols
WB protocol for PDLIM7 antibody 10221-1-APDownload protocol
IHC protocol for PDLIM7 antibody 10221-1-APDownload protocol
IF protocol for PDLIM7 antibody 10221-1-APDownload protocol
IP protocol for PDLIM7 antibody 10221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity.

Authors - Aron Gyuris
mouseIHC

J Mol Histol

Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development.

Authors - Fang Pingjuan P
humanWB

Cytotechnology

Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation.

Authors - Zhen Zhu
humanWB,IHC

Int J Mol Sci

LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells.

Authors - Huiwen Liu
humanWB

J Orthop Res

LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.

Authors - Hui Liu
  • KD Validated
humanIHC

J Endod

Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.

Authors - Wang Xiaoying X

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This antibody worked quite well in detecting PDLIM7 in mouse heart protein lysate.

  • Applications: Western Blot
  • Cell Tissue Type: Heart
FH

Joleen (Verified Customer) (06-10-2019)

The most prominent band correspond to where PDLIM7 is expected to be. But there are other surrounding background bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
PDLIM7 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10221-1-AP)