PDRG1 Polyclonal antibody

PDRG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16968-1-AP

Synonyms

C20orf126, p53 and DNA damage regulated 1, PDRG, PDRG1

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Validation Data Gallery

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  WB analysis of Jurkat using 16968-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16968-1-AP (PDRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16968-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16968-1-AP (PDRG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16968-1-AP

  Immunofluorescent analysis of HepG2 cells, using PDRG1 antibody 16968-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

16968-1-AP targets PDRG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, Artemia sinica
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDRG1 fusion protein Ag9109
• Full Name: p53 and DNA damage regulated 1
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC009334
• Gene Symbol: PDRG1
• Gene ID (NCBI): 81572
• RRID: AB_2162082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDRG1 codes for a novel protein called p53 and DNA damage-regulated protein 1. Human PDRG1 is predominantly expressed in normal testis and its expression can be induced by UV irradiation and be repressed by p53 [PMID14562055]. Recently, PDRG1 was identified as a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. Its expression was upregulated in multiple malignancies including cancers of the colon, rectum, ovary, lung, stomach, breast and uterus when compared to normal tissues [PMID: 21193842], suggesting its being a novel tumor marker that could play a role in cancer development and/or progression.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.; Authors - Paul Yenerall
mouse	IHC	DNA Repair (Amst); The influence of retinoic acid-induced differentiation on the radiation response of male germline stem cells.; Authors - Yi Zheng
human	WB	Ann Transl Med; A pan-cancer analysis of the expression and prognostic significance of PDRG1; Authors - Zhen Liu
Artemia sinica	WB	Int J Mol Sci; The Potential Roles of the Apoptosis-Related Protein PDRG1 in Diapause Embryo Restarting of Artemia sinica.; Authors - Wan Zhang