PDS5B Polyclonal antibody

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• PDS5B Polyclonal antibody (18796-1-AP)

PDS5B Polyclonal Antibody for WB, ELISA

Cat No. 18796-1-AP Copied!

Reviews (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA0979, CG008, AS3, APRIN, Androgen-induced prostate proliferative shutoff-associated protein AS3

• 

  WB analysis of HT-1080 using 18796-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 18796-1-AP (PDS5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 18796-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18796-1-AP (PDS5B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-1080 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18796-1-AP targets PDS5B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8301

  Product name: Recombinant human PDS5B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 228-529 aa of BC039256

  Sequence: AIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNEERLQVVKLLAKMFGAKDSELASQNKPLWQCYLGRFNDINVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDILLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYALQSAAGKDAAKQIAWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETTERMKCLYYLYATLDLNAVKYVSNIKFCSFHPLQYIGFYGKETTNTCILKCNLCSVNIV

• Full Name: PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
• Calculated Molecular Weight: 1391 aa, 158 kDa, 165 kDa
• Observed Molecular Weight: 165-170 kDa
• GenBank Accession Number: BC039256
• Gene Symbol: PDS5B
• Gene ID (NCBI): 23047
• RRID: AB_10642562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NTI5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review reports successful immunofluorescence staining using this antibody on mouse testis at a 1:100 dilution. The user observed very specific nuclear staining in testicular germ cells, noting that this application was not listed on the datasheet. The reviewer also used it for Western Blot at 1:1,000 dilution. Overall, the product received a 5-star rating, with the user highlighting its strong specificity and utility beyond the indicated applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Zhenmin (Verified Customer) (08-19-2020)

We tested IF staining with this primary antibody on mouse testis. It showed very specific nuclear staining in testicular germ cells. This application was not indicated in the data sheet.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1,000 (WB), 1:100 (IF)
• Cell Tissue Type: Mouse testis