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PDS5B Polyclonal antibody

PDS5B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18796-1-AP

Synonyms

APRIN, AS3, CG008, KIAA0979, PDS5B, RP1 267P19.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using PDS5B Polyclonal antibody (18796-1-AP)

    WB analysis of HT-1080 using 18796-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 18796-1-AP (PDS5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDS5B Polyclonal antibody (18796-1-AP)

    WB analysis using 18796-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18796-1-AP (PDS5B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHT-1080 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18796-1-AP is PDS5B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDS5B fusion protein Ag8301
Full Name PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
Calculated Molecular Weight 1391 aa, 158 kDa, 165 kDa
Observed Molecular Weight 165-170 kDa
GenBank Accession NumberBC039256
Gene Symbol PDS5B
Gene ID (NCBI) 23047
RRIDAB_10642562
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDS5B antibody 18796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Zhenmin (Verified Customer) (08-19-2020)

We tested IF staining with this primary antibody on mouse testis. It showed very specific nuclear staining in testicular germ cells. This application was not indicated in the data sheet.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1,000 (WB), 1:100 (IF)
  • Cell Tissue Type: Mouse testis