PDS5B Polyclonal antibody

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  WB analysis of HT-1080 using 18796-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 18796-1-AP (PDS5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18796-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18796-1-AP (PDS5B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PDS5B Polyclonal Antibody for WB, ELISA

Cat No. 18796-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA0979, CG008, AS3, APRIN, Androgen-induced prostate proliferative shutoff-associated protein AS3

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Tested Applications

• Positive WB detected in: HT-1080 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18796-1-AP targets PDS5B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8301

  Product name: Recombinant human PDS5B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 228-529 aa of BC039256

  Sequence: AIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNEERLQVVKLLAKMFGAKDSELASQNKPLWQCYLGRFNDINVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDILLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYALQSAAGKDAAKQIAWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETTERMKCLYYLYATLDLNAVKYVSNIKFCSFHPLQYIGFYGKETTNTCILKCNLCSVNIV

• Full Name: PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
• Calculated Molecular Weight: 1391 aa, 158 kDa, 165 kDa
• Observed Molecular Weight: 165-170 kDa
• GenBank Accession Number: BC039256
• Gene Symbol: PDS5B
• Gene ID (NCBI): 23047
• RRID: AB_10642562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NTI5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhenmin (Verified Customer) (08-19-2020)

We tested IF staining with this primary antibody on mouse testis. It showed very specific nuclear staining in testicular germ cells. This application was not indicated in the data sheet.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1,000 (WB), 1:100 (IF)
• Cell Tissue Type: Mouse testis