PDSS1 Polyclonal antibody

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• PDSS1 Polyclonal antibody (33109-1-AP)

• 

  WB analysis using 33109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33109-1-AP (PDSS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 33109-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PDSS1 antibody (33109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PDSS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 33109-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

All trans-polyprenyl-diphosphate synthase PDSS1, All-trans-decaprenyl-diphosphate synthase subunit 1, Decaprenyl pyrophosphate synthase subunit 1, Decaprenyl-diphosphate synthase subunit 1, EC:2.5.1.91

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33109-1-AP targets PDSS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38975

  Product name: Recombinant human PDSS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 319-415 aa of NM_014317.4

  Sequence: MGKPTSADLKLGLATGPVLFACQQFPEMNAMIMRRFSLPGDVDRARQYVLQSDGVQQTTYLAQQYCHEAIREISKLRPSPERDALIQLSEIVLTRDK

• Full Name: prenyl (decaprenyl) diphosphate synthase, subunit 1
• Calculated Molecular Weight: 46kDa，415aa
• Observed Molecular Weight: 34-46 kDa
• GenBank Accession Number: NM_014317.4
• Gene Symbol: PDSS1
• Gene ID (NCBI): 23590
• RRID: AB_3742859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5T2R2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDSS1 is also known as DPS1 or TPRT. The protein encoded by this gene is an enzyme that elongates the prenyl side-chain of coenzyme Q (also known as ubiquinone), one of the key elements in the respiratory chain. It catalyses the formation of all-trans polyprenyl pyrophosphates from isopentyl diphosphate during the assembly of polyisoprenoid side chains - the first step in coenzyme Q biosynthesis. PDSS1 forms a functional heterodimer with the PDSS2 subunit, which is responsible for catalysing the chain elongation reaction of isopentenyl diphosphate to generate isoprenoid precursors of varying lengths, such as farnesyl diphosphate and geranylgeranyl diphosphate. These products are fundamental to the synthesis of essential molecules such as cholesterol, ubiquinone, heme A and protein isoprenylation modifications, as well as being vital for other biological processes. Therefore, PDSS1 directly influences key physiological processes in cells by regulating isoprenoid synthesis, including energy metabolism, membrane stability, signal transduction, and antioxidant defence.