PDXDC1 Polyclonal antibody

PDXDC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21021-1-AP

Synonyms

Pyridoxal-dependent decarboxylase domain-containing protein 1, LP8165, KIAA0251, EC:4.1.1.-

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Validation Data Gallery

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  WB analysis using 21021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21021-1-AP

  mouse testis lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21021-1-AP

  IP result of anti-PDXDC1 (IP:21021-1-AP, 4ug; Detection:21021-1-AP 1:500) with HepG2 cells lysate 1680 ug.

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  IHC staining of human hepatocirrhosis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21021-1-AP

  Immunofluorescent analysis of HepG2 cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 21021-1-AP

  Immunofluorescent analysis of Hela cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue, HeLa cells, SGC-7901 cells, rat testis tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human hepatocirrhosis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

21021-1-AP targets PDXDC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDXDC1 fusion protein Ag15283
• Full Name: pyridoxal-dependent decarboxylase domain containing 1
• Calculated Molecular Weight: 788 aa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC033748
• Gene Symbol: PDXDC1
• Gene ID (NCBI): 23042
• RRID: AB_10695768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDXDC1 (pyridoxal-dependent decarboxylase domain containing 1) is a putative enzyme that could metabolize catecholamine neurotransmitters (PMID:28485732). With prior evidence for involvement with glioblastoma from other previously reported experimental settings, and contains the lead single nucleotide polymorphism (rs3198697) from the linkage analysis of the chromosome 16 region (PMID:32644145). Moreover, PDXDC1 is involved in the catalysis of the nonhydrolytic addition or removal of a carboxyl group to or from a compound (PMID:29277971).

Publications

Species	Application	Title
mouse	IHC	J Mol Med (Berl); Identification of PDXDC1 as a novel pleiotropic susceptibility locus shared between lumbar spine bone mineral density and birth weight.; Authors - Yu-Qian Song
mouse	WB	Cell Mol Life Sci; Protease-independent control of parthanatos by HtrA2/Omi; Authors - Jonas Weiß