PDXK Polyclonal antibody

PDXK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 15309-1-AP

Synonyms

C21orf124, C21orf97, PDXK, PKH, PNK, PRED79, Pyridoxal kinase, Pyridoxine kinase

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Validation Data Gallery

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  WB analysis using 15309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15309-1-AP (PDXK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15309-1-AP

  IP result of anti-PDXK (IP:15309-1-AP, 4ug; Detection:15309-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human urothelial carcinoma using 15309-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 15309-1-AP (PDXK antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 15309-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 15309-1-AP (PDXK antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, mouse liver tissue, mouse spinal cord, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15309-1-AP targets PDXK in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDXK fusion protein Ag7451
• Full Name: pyridoxal (pyridoxine, vitamin B6) kinase
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 40 kDa, 35 kDa
• GenBank Accession Number: BC000123
• Gene Symbol: PDXK
• Gene ID (NCBI): 8566
• RRID: AB_2252232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDXK encodes for a pyridoxal kinase, which converts inactive B6 vitamers to the active cofactor pyridoxal 5'-phosphate (PLP).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Natural product P57 induces hypothermia through targeting pyridoxal kinase; Authors - Ruina Wang
mouse	IF,IHC	Cell Rep; Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease; Authors - Min Yu
human	WB	Drug Deliv Transl Res; Delivery of exogenous miR-19b by Wharton's Jelly Mesenchymal Stem Cells attenuates transplanted kidney ischemia/reperfusion injury by regulating cellular metabolism; Authors - Xiaoqiang Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karan (Verified Customer) (12-02-2022)

Works well for Western Blotting. There is one very strong specific band and 2 weak non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: iPSC Neurons