Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, mouse liver tissue, mouse spinal cord, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15309-1-AP targets PDXK in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7451 Product name: Recombinant human PDXK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC000123 Sequence: MEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQELYEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMERIRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL Predict reactive species |
| Full Name | pyridoxal (pyridoxine, vitamin B6) kinase |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 40 kDa, 35 kDa |
| GenBank Accession Number | BC000123 |
| Gene Symbol | PDXK |
| Gene ID (NCBI) | 8566 |
| RRID | AB_2252232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00764 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDXK encodes for a pyridoxal kinase, which converts inactive B6 vitamers to the active cofactor pyridoxal 5'-phosphate (PLP).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDXK antibody 15309-1-AP | Download protocol |
| IP protocol for PDXK antibody 15309-1-AP | Download protocol |
| WB protocol for PDXK antibody 15309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol A pan-cancer analysis of the expression and molecular mechanism of DHX9 in human cancers | ||
Cell Rep Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease | ||
Drug Deliv Transl Res Delivery of exogenous miR-19b by Wharton's Jelly Mesenchymal Stem Cells attenuates transplanted kidney ischemia/reperfusion injury by regulating cellular metabolism | ||
Nat Commun SLC25A38 is required for mitochondrial pyridoxal 5'-phosphate (PLP) accumulation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Karan (Verified Customer) (12-02-2022) | Works well for Western Blotting. There is one very strong specific band and 2 weak non-specific bands.
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