PDXP Polyclonal antibody

PDXP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27490-1-AP

Synonyms

CIN, PDXP, PLP, PLP phosphatase, PLPP

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Validation Data Gallery

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  WB analysis using 27490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27490-1-AP (PDXP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27490-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27490-1-AP (PDXP antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27490-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27490-1-AP (PDXP antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 27490-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27490-1-AP (PDXP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27490-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27490-1-AP (PDXP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Neuro-2a cells, mouse brain tissue
• Positive IHC detected in: human liver tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

27490-1-AP targets PDXP in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDXP fusion protein Ag26592
• Full Name: pyridoxal (pyridoxine, vitamin B6) phosphatase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC064922
• Gene Symbol: PDXP
• Gene ID (NCBI): 57026
• RRID: AB_2880887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDXP, also named CIN, belongs to the HAD-like hydrolase superfamily. As a pyridoxal phosphate (PLP) phosphatase, PDXP can catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. PDXP is highly expressed in all the regions of central nerve system except the spinal cord. PDXP is also expressed at high level in liver and testis.

Publications

Species	Application	Title
human,mouse	WB,IHC	Mol Carcinog; p110CUX1 promotes acute myeloid leukemia progression via regulating pyridoxal phosphatase expression and activating PI3K/AKT/mTOR signaling pathway; Authors - Hongyan Zhang ; KD Validated