PDZK1 Polyclonal antibody

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• PDZK1 Polyclonal antibody (10507-2-AP)

PDZK1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10507-2-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

CAP70, CFTR-associated protein of 70 kDa, Na(+)/H(+) exchange regulatory cofactor NHE-RF3, Na/Pi cotransporter C-terminal-associated protein 1, NaPi-Cap1

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  WB analysis of mouse kidney using 10507-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10507-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 10507-2-AP

  IP result of anti-PDZK1 (IP:10507-2-AP, 3ug; Detection:10507-2-AP 1:500) with HEK-293 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10507-2-AP targets PDZK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0787

  Product name: Recombinant human PDZK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC006496

  Sequence: MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVKEGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFLLVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEAGLKNNDLVVAVNGESVETLD

• Full Name: PDZ domain containing 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC006496
• Gene Symbol: PDZK1
• Gene ID (NCBI): 5174
• RRID: AB_2299495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T2W1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDZK1 is a PDZ domain-containing membrane scaffold protein widely expressed in a variety of epithelial cells. It plays a key role in cell signal transduction and ion channel regulation by interacting with a variety of ion channels, transporters, and receptors. PDZK1 simultaneously anchors multiple membrane proteins through its PDZ domain to regulate membrane expression and activity. PDZK1 maintains cholesterol homeostasis and participates in the regulation of the urate/anion exchanger (URAT1) in renal tubular uric acid reabsorption.

Publications

What published studies show

The anti-PDZK1 antibody (Cat# 10507-2-AP) has 13 total citations. These appear in journals including Bone Research, Advanced Science, Molecular Medicine, Biochemical Pharmacology, Oncotarget, Scientific Reports, Cell Division, Experimental Eye Research, Food Chemistry X, Nutrients, Aging, and Clinical and Experimental Pharmacology and Physiology. Research fields span osteoarthritis, diabetic retinopathy, hyperuricemia, breast cancer, hepatocellular carcinoma, glioma, renal fibrosis, ovarian cancer drug resistance, and vascular hypertension.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	Bone Res; PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function; Authors - Yan Shao
human	WB,CoIP	Adv Sci (Weinh); PDZK1 Protects Against RPE Senescence by Targeting the 14-3-3ε-mTOR Axis to Attenuate Early Diabetic Retinopathy; Authors - Jian Zhao
mouse	WB,IHC	Food Chem X; The mature stem of green Asparagus as a potential dietary supplement for hyperuricemia: Asparagus ameliorates hyperuricemia by regulating hepatic uric acid metabolism and renal uric acid excretion.; Authors - Yinjie Zhang
human	IHC	Mol Med; Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37.; Authors - Hogyoung Kim
human	IHC	Mol Med; PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth.; Authors - Kim Hogyoung H
human,mouse	WB	Biochem Pharmacol; GRP/GRPR signaling pathway aggravates hyperuricemia-induced renal inflammation and fibrosis via ABCG2-dependent mechanisms.; Authors - Hao-Lu Sun