PELI2 Polyclonal antibody

PELI2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16097-1-AP

Synonyms

RING-type E3 ubiquitin transferase pellino homolog 2, Protein pellino homolog 2, Pellino-2, Pellino 2, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis using 16097-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16097-1-AP (PELI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 16097-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16097-1-AP (PELI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 16097-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16097-1-AP (PELI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16097-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PELI2 antibody (16097-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16097-1-AP targets PELI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PELI2 fusion protein Ag8940
• Full Name: pellino homolog 2 (Drosophila)
• Calculated Molecular Weight: 420 aa, 46 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC009476
• Gene Symbol: PELI2
• Gene ID (NCBI): 57161
• RRID: AB_2878218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.