PELP1 Polyclonal antibody, PBS Only

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• PELP1 Polyclonal antibody, PBS Only (30135-1-PBS)

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  WB analysis using 30135-1-AP (same clone as 30135-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30135-1-AP (PELP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 30135-1-AP (same clone as 30135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 30135-1-AP (same clone as 30135-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PELP1 antibody (30135-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

PELP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 30135-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

30135-1-PBS targets PELP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32720

  Product name: Recombinant human PELP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1000-1130 aa of BC069058

  Sequence: EPEPGLLLEVEEPGTEEERGADTAPTLAPEALPSQGEVEREGESPAAGPPPQELVEEEPSAPPTLLEEETEDGSDKVQPPPETPAEEEMETETEAEALQEKEQDDTAAMLADFIDCPPDDEKPPPPTEPDS

• Full Name: proline, glutamate and leucine rich protein 1
• Calculated Molecular Weight: 1130 aa, 120 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC069058
• Gene Symbol: PELP1
• Gene ID (NCBI): 27043
• RRID: AB_2935519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZL8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.