PEMT Polyclonal antibody

PEMT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25916-1-AP

Synonyms

PEAMT, PEMPT, PEMT, PEMT2, PNMT

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Validation Data Gallery

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  WB analysis of rat liver using 25916-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 25916-1-AP (PEMT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 25916-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25916-1-AP (PEMT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 25916-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human testis using 25916-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 25916-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat liver tissue, mouse liver tissue
• Positive IHC detected in: human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25916-1-AP targets PEMT in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEMT fusion protein Ag5872
• Full Name: phosphatidylethanolamine N-methyltransferase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC050593
• Gene Symbol: PEMT
• Gene ID (NCBI): 10400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu