PEMT Polyclonal antibody

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• PEMT Polyclonal antibody (25916-1-AP)

PEMT Polyclonal Antibody for IHC, ELISA

Cat No. 25916-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, ELISA

Synonyms

EC:2.1.1.17, PEAMT, PEMPT, PEMT2, Phosphatidylethanolamine N-methyltransferase

• 

  IHC staining of human liver using 25916-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:400 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 25916-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:400 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 25916-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 25916-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25916-1-AP targets PEMT in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5872

  Product name: Recombinant human PEMT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-236 aa of BC050593

  Sequence: MKRSGNPGAEVTNSSVAGPDCCGGLGNIDFRQADFCVMTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS

• Full Name: phosphatidylethanolamine N-methyltransferase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC050593
• Gene Symbol: PEMT
• Gene ID (NCBI): 10400
• RRID: AB_3669508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu
rat	IHC	Small Methods; Integrating IDEAL-IQ Sequence with Mass Spectrometry Imaging for Profiling Lipid-Related Metabolic Reprogramming of Acute Kidney Injury.; Authors - Huayu Zhao
		Int J Biol Macromol; Atractylodes lancea (Thunb.) DC polysaccharide alleviates MASH by regulating the 1‑carbon cycle through intestinal flora remodelling.; Authors - Dajin Pi