Proenkephalin-A Polyclonal antibody

Proenkephalin-A Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 13531-1-AP

Synonyms

Opioid growth factor, PENK, proenkephalin, Proenkephalin A, Proenkephalin-A

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Validation Data Gallery

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  IHC staining of human testis using 13531-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13531-1-AP (Proenkephalin-A antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13531-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13531-1-AP (Proenkephalin-A antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13531-1-AP targets Proenkephalin-A in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Proenkephalin-A fusion protein Ag4428
• Full Name: proenkephalin
• Calculated Molecular Weight: 267 aa, 31 kDa
• GenBank Accession Number: BC032505
• Gene Symbol: PENK
• Gene ID (NCBI): 5179
• RRID: AB_2161510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proenkephalin-A (PENK) is also named as Synenkephalin, Met-enkephalin and Opioid growth factor (OGF), and belongs to the opioid neuropeptide precursor family. PENK is a neuropeptide that competes with and mimics the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (PMID:7057924). PENK hypermethylation is also associated with bladder cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer (PMID:36403035).