PEPD Polyclonal antibody

• Home
• Products
• Primary Antibodies
• PEPD Polyclonal antibody (12218-1-AP)

• Featured Product
• KD/KO Validated

PEPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12218-1-AP Copied!

Publications (4)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.13.9, PRD, PROLIDASE, Xaa-Pro dipeptidase, X-Pro dipeptidase

• 

  WB analysis using 12218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 12218-1-AP

  WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 12218-1-AP

  WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skin using 12218-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MDA-MB-453s using 12218-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 12218-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12218-1-AP (PEPD antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 12218-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PEPD antibody (12218-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for PEPD in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, MDA-MB-453s cells, mouse skin tissue, HeLa cells, HepG2 cells, SH-SY5Y cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:100-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12218-1-AP targets PEPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2842

  Product name: Recombinant human PEPD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 192-439 aa of BC015027

  Sequence: LEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREV

• Full Name: peptidase D
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC015027
• Gene Symbol: PEPD
• Gene ID (NCBI): 5184
• RRID: AB_2161517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12955
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

Publications

What published studies show

The PEPD Polyclonal antibody (Cat# 12218-1-AP) has 4 citations published in Nature Communications, Redox Biology, Archives of Medical Research, and BMC Medical Genetics. The research fields span developmental biology (miRNA regulation in locust egg hatching), redox biology and p53 signaling, cardiovascular disease (aortic stiffness in coronary artery disease), and genetic susceptibility to abdominal aortic aneurysms.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA; Authors - Ya Nan Zhu
human	WB	Redox Biol; ReDisulphID: A discovery platform for thiol redox sensors identifies a druggable site regulating p53 activation.; Authors - Pierre Coleman
human	IHC	Arch Med Res; Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.; Authors - Aydemir Koçarslan
human	IHC	BMC Med Genet; Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19.; Authors - Lillvis John H JH