PER1 Recombinant monoclonal antibody, PBS Only

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  IF Staining of MCF-7 using 84730-5-RR (same clone as 84730-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84730-5-RR (same clone as 84730-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84730-5-RR (same clone as 84730-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PER1 Recombinant antibody (84730-5-RR, Clone:242096E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84730-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84730-5-RR against Human PER1 were performed. The affinity constant is 0.802 nM.

PER1 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84730-5-PBS

Clone No.242096E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242096E3, hPER, hPER1, KIAA0482, PER

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Product Information

84730-5-PBS targets PER1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4249

  Product name: Recombinant human PER1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC028207

  Sequence: MSGPLEGADGGGDPRPGESFCPGGVPSPGPPQHRPCPGPSLADDTDANSNGSSGNESNGHESRGASQRSSHSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMTALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCSMDMSTYTLEELEHITSEYTLQNQDTFSVAVSFLTGRIVYISEQAAVLLRCKRDVFRGTRFSELLAPQDVGVFYGSTAPSRLPTWGTGASAGSGLRDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAE

• Full Name: period homolog 1 (Drosophila)
• Calculated Molecular Weight: 1290 aa, 136 kDa
• GenBank Accession Number: BC028207
• Gene Symbol: PER1
• Gene ID (NCBI): 5187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15534
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143].