PER3 Polyclonal antibody

PER3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 12550-1-AP

Synonyms

period homolog 3 (Drosophila), hPER3, GIG13, Cell growth-inhibiting gene 13 protein

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Validation Data Gallery

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  WB analysis of HeLa using 12550-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12550-1-AP (PER3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12550-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12550-1-AP (PER3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12550-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12550-1-AP (PER3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

12550-1-AP targets PER3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PER3 fusion protein Ag3400
• Full Name: period homolog 3 (Drosophila)
• Calculated Molecular Weight: 1201 aa, 132 kDa
• Observed Molecular Weight: 132 kDa
• GenBank Accession Number: BC026102
• Gene Symbol: PER3
• Gene ID (NCBI): 8863
• RRID: AB_2161814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The suprachiasmatic nuclei (SCN) of the anterior hypothalamus function as a circadian clock in the mammalian brain, generating circadian rhythms in physiology and behavior. The period homolog genesPer3 is an important component of the circadian clock system. PER3 plays a role in cell proliferation, apoptosis,DNA damage response, cell cycle control, and treatment response of chemotherapy agents in cancers.

Publications

Species	Application	Title
rat	WB	Arthritis Res Ther; Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats.; Authors - Xiaopeng Song
human	WB	Int J Biochem Cell Biol; TFEB Regulates PER3 Expression via Glucose-dependent Effects on CLOCK/BMAL1.; Authors - Wenwen Luo
human	WB	Biochem Biophys Res Commun; Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.; Authors - Xiaomeng Shen
human	WB	Adv Sci (Weinh); The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis; Authors - Tianshu Yang
mouse	WB	Anesth Analg; Sleep Fragmentation, Electroencephalographic Slowing, and Circadian Disarray in a Mouse Model for Intensive Care Unit Delirium; Authors - Elzbieta Dulko
human	WB	Cancer Lett; Forkhead Box Protein FOXK1 Disrupts the Circadian Rhythm to Promote Breast Tumorigenesis in Response to Insulin Resistance; Authors - Zhaohan Zhang