Tested Applications
| Positive WB detected in | Daudi cells, HSC-T6 cells, Ramos cells, Jurkat cells, K-562 cells, HuH-7 cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68482-1-Ig targets PERK/EIF2AK3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18254 Product name: Recombinant human EIF2AK3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 767-1116 aa of BC126354 Sequence: TDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2-alpha kinase 3 |
| Calculated Molecular Weight | 1116 aa, 125 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC126354 |
| Gene Symbol | PERK |
| Gene ID (NCBI) | 9451 |
| RRID | AB_3085193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NZJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PERK/EIF2AK3 antibody 68482-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PERK/EIF2AK3 Monoclonal antibody (3G3F2), catalog number 68482-1-Ig, has 18 citations published in journals including Cell Commun Signal, Sci China Life Sci, Free Radic Biol Med, FASEB J, Sci Rep, and Adv Sci. Research fields span endoplasmic reticulum stress/UPR signaling, NAFLD/MASLD, cancer (breast, glioblastoma, head and neck SCC), diabetic complications, myopia, traumatic brain injury, atherosclerosis, ulcerative colitis, and graft-versus-host disease. Applications are primarily WB and IF across human, mouse, rat, and pig species.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal Regulatory role of sirtuin-1 by targeting MDM2 to mitochondria-associated membranes formation in the treatment of NAFLD. | ||
Sci China Life Sci SPHK1 deficiency promotes intestinal homeostasis by ameliorating ER stress-induced gastrointestinal injury during murine graft-versus-host disease. | ||
Free Radic Biol Med Ultrasound-Responsive Taurine Lipid Nanoparticles Attenuate Oxidative Stress and Promote Macrophage Polarization for Diabetic Wound Healing | ||
Ecotoxicol Environ Saf Changes of N(6)-methyladenosine modification and unfolded protein response in renal cell injury induced by cadmium. | ||
Cancer Gene Ther N6-methyladenosine modification of RIMS binding protein 2 promotes head and neck squamous cell carcinoma proliferation and radiotherapy tolerance through endoplasmic reticulum stress | ||
Commun Biol CCR7-PERK-CREB3L1 signaling mediates tumor-associated macrophages-derived SPP1 promotion of oral squamous cell carcinoma. |
