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PERK/EIF2AK3 Recombinant monoclonal antibody, PBS Only (88220-2-PBS)

PERK/EIF2AK3 Recombinant monoclonal antibody, PBS Only

Name: PERK/EIF2AK3 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 88220-2-PBS-100UG

PERK/EIF2AK3 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA

Cat No. 88220-2-PBS

Clone No.260327C7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

EIF2AK3, PERK, EC:2.7.11.1, EIF2AK 3, Eukaryotic translation initiation factor 2-alpha kinase 3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWBIPFC (Intra)

WB analysis using 88220-2-RR (same clone as 88220-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 88220-2-RR (PERK/EIF2AK3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88220-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 88220-2-RR (same clone as 88220-2-PBS)
IP result of anti-PERK/EIF2AK3 (IP:88220-2-RR, 4ug; Detection:88220-2-RR 1:800) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 88220-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 88220-2-RR (same clone as 88220-2-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug PERK/EIF2AK3 Recombinant monoclonal antibody (88220-2-RR, Clone:260327C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 88220-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 HeLa cells were intracellularly stained with 0.25 ug PERK/EIF2AK3 Recombinant monoclonal antibody (88220-2-RR, Clone:260327C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 88220-2-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

88220-2-PBS targets PERK/EIF2AK3 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag18254 Product name: Recombinant human EIF2AK3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 767-1116 aa of BC126354 Sequence: TDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN Predict reactive species
Full Name	eukaryotic translation initiation factor 2-alpha kinase 3
Calculated Molecular Weight	1116 aa, 125 kDa
Observed Molecular Weight	140 kDa
GenBank Accession Number	BC126354
Gene Symbol	PERK
Gene ID (NCBI)	9451
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NZJ5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170 kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).