Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13669-1-AP targets PEX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4623 Product name: Recombinant human PEX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 927-1283 aa of BC035575 Sequence: IRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA Predict reactive species |
| Full Name | peroxisomal biogenesis factor 1 |
| Calculated Molecular Weight | 1283 aa, 143 kDa |
| Observed Molecular Weight | 143 kDa |
| GenBank Accession Number | BC035575 |
| Gene Symbol | PEX1 |
| Gene ID (NCBI) | 5189 |
| RRID | AB_2162104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43933 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Peroxin (PEX) proteins generate and maintain peroxisomes. The peroxin-1 (PEX1) ATPase facilitates the recycling of the peroxisome matrix protein receptor PEX5 and is the most commonly affected peroxin in human peroxisome biogenesis disorders (PMID: 28600347). PEX1 and PEX6 are the only members of the AAA family (for ATPases associated with diverse cellular) activities implicated in peroxisome biogenesis and are closely related to p97, which functions in endoplasmic reticulum-associated protein degradation to retrotranslocate endoplasmic reticulum proteins to the cytosol (PMID: 9695811; 11740563).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PEX1 antibody 13669-1-AP | Download protocol |
| IHC protocol for PEX1 antibody 13669-1-AP | Download protocol |
| WB protocol for PEX1 antibody 13669-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PEX1 Polyclonal antibody (Cat# 13669-1-AP) has 16 citations. These appear in journals including Autophagy, Cell Death & Differentiation, Cell Communication & Signaling, Cell Death Discovery, EMBO Journal, Cells, International Dental Journal, Molecular Therapy Methods & Clinical Development, European Journal of Human Genetics, Molecular Genetics & Metabolism, Experimental Eye Research, Journal of Cellular Biochemistry, PLoS One, and Biological Chemistry. Research fields span peroxisome biogenesis and Zellweger spectrum disorders, autophagy/pexophagy, cancer, hearing loss, retinopathy, and inflammation.
| Species | Application | Title |
|---|---|---|
Autophagy AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. | ||
Autophagy Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts. | ||
Autophagy The ER protein CANX (calnexin)-mediated autophagy protects against alzheimer disease | ||
Cell Death Differ MARCH8/NSUN6/ROS-mediated DNA damage positive feedback loop regulates cisplatin resistance in osteosarcoma | ||
Cell Commun Signal TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions | ||
Cell Death Discov A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence. |
