PEX11G Polyclonal antibody

PEX11G Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15744-1-AP

Synonyms

Peroxin 11C, PEX11 gamma, PEX11C, PEX11G, Protein PEX11 homolog gamma

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Validation Data Gallery

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  WB analysis of human testis using 15744-1-AP

  human testis tissue was subjected to SDS PAGE followed by western blot with 15744-1-AP (PEX11G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15744-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15744-1-AP (PEX11G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15744-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15744-1-AP (PEX11G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15744-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15744-1-AP (PEX11G antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15744-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15744-1-AP (PEX11G antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-12 cells, human testis tissue, mouse testis tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15744-1-AP targets PEX11G in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX11G fusion protein Ag8179
• Full Name: peroxisomal biogenesis factor 11 gamma
• Calculated Molecular Weight: 241 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC008780
• Gene Symbol: PEX11G
• Gene ID (NCBI): 92960
• RRID: AB_2162132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Med Genet; A novel defect of peroxisome division due to a homozygous non-sense mutation in the PEX11β gene.; Authors - Ebberink Merel S MS