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  • KD/KO Validated

PEX16 Polyclonal antibody

PEX16 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14816-1-AP

Synonyms

Peroxin 16, PEX16



Tested Applications

Positive WB detected inhuman liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14816-1-AP targets PEX16 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Arabidopsis, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX16 fusion protein Ag6566
Full Name peroxisomal biogenesis factor 16
Calculated molecular weight 39 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000467
Gene symbol PEX16
Gene ID (NCBI) 9409
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).

Protocols

Product Specific Protocols
WB protocol for PEX16 antibody 14816-1-APDownload protocol
IF protocol for PEX16 antibody 14816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ArabidopsisWB

J Integr Plant Biol

PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants.

Authors - Sarah E Burkhart
mouseWB

Methods Mol Biol

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

Authors - Miriam J Schönenberger
mouseWB

Biochim Biophys Acta

Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.

Authors - Kovacs Werner J WJ
humanWB

Int J Mol Sci

Knockdown of PEX16 Induces Autophagic Degradation of Peroxisomes.

Authors - Xiaofan Wei
  • KD Validated
mouseWB

PLoS One

Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell.

Authors - Yizhu Mu
mouseWB

Biochim Biophys Acta

Critical role of the peroxisomal protein PEX16 in white adipocyte development and lipid homeostasis.

Authors - Dina C Hofer
  • KD Validated