|Positive WB detected in||human liver tissue|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14816-1-AP targets PEX16 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Arabidopsis, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PEX16 fusion protein Ag6566|
|Full Name||peroxisomal biogenesis factor 16|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||BC000467|
|Gene ID (NCBI)||9409|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).
J Integr Plant Biol
PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants.
Methods Mol Biol
Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.
Biochim Biophys Acta
Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.
Int J Mol Sci
Knockdown of PEX16 Induces Autophagic Degradation of Peroxisomes.
Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell.
Biochim Biophys Acta
Critical role of the peroxisomal protein PEX16 in white adipocyte development and lipid homeostasis.