PEX16 Recombinant monoclonal antibody

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• PEX16 Recombinant monoclonal antibody (86887-1-RR)

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  WB analysis using 86887-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86887-1-RR (PEX16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86887-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86887-1-RR against Human PEX16 were performed. The affinity constant is 5.30 nM.

PEX16 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86887-1-RR

Clone No.251835F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Peroxin 16, Peroxin-16, PEX

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, A549 cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86887-1-RR targets PEX16 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6566

  Product name: Recombinant human PEX16 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 132-346 aa of BC000467

  Sequence: KAGLQTSPPIVPLDRETQAQPPDGDHSPGNHEQSYVGKRSNRVVRTLQNTPSLHSRHWGAPQQREGRQQQHHEELSATPTPLGLQETIAEFLYIARPLLHLLSLGLWGQRSWKPWLLAGVVDVTSLSLLSDRKGLTRRERRELRRRTILLLYYLLRSPFYDRFSEARILFLLQLLADHVPGVGLVTTSQRAASPCLPARPHTQPWSPPAFLPGHP

• Full Name: peroxisomal biogenesis factor 16
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000467
• Gene Symbol: PEX16
• Gene ID (NCBI): 9409
• RRID: AB_3745188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y5Y5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).