PEX3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PEX3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10946-1-AP

Synonyms

Cell and organelle markers, Peroxin 3, Peroxisome Marker, PEX3, TRG18

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Validation Data Gallery

• 

  WB analysis of HeLa using 10946-1-AP

  WB result of PEX3 antibody (10946-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX3 transfected HeLa cells.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IF: See 1 publications below

Product Information

10946-1-AP targets PEX3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, arabidopsis
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX3 fusion protein Ag1384
• Full Name: peroxisomal biogenesis factor 3
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC014551
• Gene Symbol: PEX3
• Gene ID (NCBI): 8504
• RRID: AB_2162392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 3, also known as PEX3 (peroxisomal biogenesis factor 3) or TRG18, is a 373 amino acid multi-pass membrane protein that anchored in the peroxisomal membrane and essential for peroxisome biogenesis and integrity. Additionally, Peroxin 3 acts as a docking factor for Peroxin 19 and is required for the import of peroxisomal proteins. Defects in the gene encoding Peroxin 3 are the cause of peroxisome biogenesis disorder complementation group 12 (PBD-CG12) and Zellweger syndrome (ZwS), both of which arise from a failure of peroxisomal protein import.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter
human	WB	Cell Death Differ; Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.; Authors - Weiwei Cui
mouse	WB	Kidney360; Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism.; Authors - Takeshi Terabayashi
mouse	WB	Methods Mol Biol; Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.; Authors - Miriam J Schönenberger
mouse	WB	Biochim Biophys Acta; Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.; Authors - Kovacs Werner J WJ
Arabidopsis	WB	J Integr Plant Biol; PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants.; Authors - Sarah E Burkhart

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (07-14-2022)

BSA block in TBS

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293

PEX3 Antibody Western Blot validation (1:500 dilution) in HEK293 (Cat no:10946-1-AP)