Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, HepG2 cells, PC-12 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30424-1-AP targets PEX3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31516 Product name: Recombinant human PEX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 37-140 aa of BC014551 Sequence: IREIQEREAAEYIAQARRQYHFESNQRTCNMTVLSMLPTLREALMQQLNSESLTALLKNRPSNKLEIWEDLKIISFTRSTVAVYSTCMLVVLLRVQLNIIGGYI Predict reactive species |
| Full Name | peroxisomal biogenesis factor 3 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC014551 |
| Gene Symbol | PEX3 |
| Gene ID (NCBI) | 8504 |
| RRID | AB_3086310 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56589 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 3, also known as PEX3 (peroxisomal biogenesis factor 3) or TRG18, is a 373 amino acid multi-pass membrane protein that anchored in the peroxisomal membrane and essential for peroxisome biogenesis and integrity. Additionally, Peroxin 3 acts as a docking factor for Peroxin 19 and is required for the import of peroxisomal proteins. Defects in the gene encoding Peroxin 3 are the cause of peroxisome biogenesis disorder complementation group 12 (PBD-CG12) and Zellweger syndrome (ZwS), both of which arise from a failure of peroxisomal protein import.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PEX3 antibody 30424-1-AP | Download protocol |
| IHC protocol for PEX3 antibody 30424-1-AP | Download protocol |
| WB protocol for PEX3 antibody 30424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PEX3 Polyclonal antibody (Cat# 30424-1-AP) has 3 total citations. These appear in J Immunother Cancer, Biomedicines, and MethodsX. The research fields span immunotherapy and colorectal cancer (macrophage CCL7 regulation), lipid metabolism in androgenetic alopecia, and mitochondrial purification methodology. Applications cited include WB and IF, with reactivity to human and mouse.
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Macrophage CCL7 promotes resistance to immunotherapy for colorectal cancer by regulating the infiltration of macrophages and CD8+ T cells | ||
Biomedicines Integrated Multi-Omics Analysis Reveals Dysregulated Lipid Metabolism as a Novel Mechanism in Androgenetic Alopecia. | ||
MethodsX Purification of murine cortex mitochondria using a modified magnetic bead isolation method. |
