Tested Applications
| Positive WB detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20614-1-AP targets PEX7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14666 Product name: Recombinant human PEX7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-323 aa of BC006268 Sequence: GEQLVVSGSWDQTVKLWDPTVGKSLCTFRGHESIIYSTIWSPHIPGCFASASGDQTLRIWDVKAAGVRIVIPAHQAEILSCDWCKYNENLLVTGAVDCSLRGWDLRNVRQPVFELLGHTYAIRRVKFSPFHASVLASCSYDFTVRFWNFSKPDSLLETVEHHTEFTCGLDFSLQSPTQVADCSWDETIKIYDPACLTIPA Predict reactive species |
| Full Name | peroxisomal biogenesis factor 7 |
| Calculated Molecular Weight | 323 aa, 36 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC006268 |
| Gene Symbol | PEX7 |
| Gene ID (NCBI) | 5191 |
| RRID | AB_10694826 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00628 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PEX7, also known as peroxisomal targeting signal 2 (PTS2) receptor, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively. PEX7 plays an essential role in peroxisomal protein import by binding to the N-terminal PTS2-type peroxisomal targeting signal. PEX7 defects are associated with peroxisome biogenesis disorder complementation group 11 (PBD-CG11), rhizomelic chondrodysplasia punctata type 1 (RCDP1) and Refsum disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX7 antibody 20614-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PEX7 Polyclonal antibody (Cat# 20614-1-AP) has 4 citations published in Nature Cell Biology, Journal of Lipid Research, PLoS Genetics, and PLoS ONE. These studies span research fields including peroxisomal protein import, ether-glycerophospholipid synthesis after traumatic brain injury, PTS2 enzyme localization and lipid metabolic abnormalities, and peroxisome-related inflammatory responses. All citations used the antibody for Western blot applications.
| Species | Application | Title |
|---|---|---|
PLoS Genet Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility. | ||
PLoS One Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell. |
