PEX7 Polyclonal antibody

PEX7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20614-1-AP

Synonyms

Peroxin 7, PEX7, PTS2 receptor, PTS2R, RCDP1, RD

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 20614-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20614-1-AP (PEX7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 20614-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20614-1-AP (PEX7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 20614-1-AP is PEX7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX7 fusion protein Ag14666
• Full Name: peroxisomal biogenesis factor 7
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC006268
• Gene Symbol: PEX7
• Gene ID (NCBI): 5191
• RRID: AB_10694826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEX7, also known as peroxisomal targeting signal 2 (PTS2) receptor, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively. PEX7 plays an essential role in peroxisomal protein import by binding to the N-terminal PTS2-type peroxisomal targeting signal. PEX7 defects are associated with peroxisome biogenesis disorder complementation group 11 (PBD-CG11), rhizomelic chondrodysplasia punctata type 1 (RCDP1) and Refsum disease.

Publications

Species	Application	Title
mouse	WB	PLoS Genet; Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.; Authors - Mizuno Yumi Y
mouse	WB	PLoS One; Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell.; Authors - Yizhu Mu