PFAS Recombinant monoclonal antibody

• 

  IF Staining of HepG2 using 83074-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PFAS antibody (83074-3-RR, Clone: 230422D12 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  Affinity and Kinetic Characterization of 83074-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83074-3-RR against Human PFAS were performed. The affinity constant is 0.58 nM.

PFAS Uni-rAb^® Recombinant Antibody for IF/ICC, ELISA

Cat No. 83074-3-RR

Clone No.230422D12

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Synonyms

FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83074-3-RR targets PFAS in IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20162

  Product name: Recombinant human PFAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 989-1338 aa of BC167158

  Sequence: MVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLSINARNWTLEGSC

• Full Name: phosphoribosylformylglycinamidine synthase
• Calculated Molecular Weight: 1338 aa, 145 kDa
• GenBank Accession Number: BC167158
• Gene Symbol: PFAS
• Gene ID (NCBI): 5198
• RRID: AB_3670794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15067
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.