PFDN1 Polyclonal antibody

PFDN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11033-2-AP

Synonyms

PDF, PFD1, PFDN1, prefoldin subunit 1

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Validation Data Gallery

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  WB analysis of human brain using 11033-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11033-2-AP (PFDN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11033-2-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11033-2-AP (PFDN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11033-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11033-2-AP (PFDN1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, human colon tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

11033-2-AP targets PFDN1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFDN1 fusion protein Ag1506
• Full Name: prefoldin subunit 1
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC011869
• Gene Symbol: PFDN1
• Gene ID (NCBI): 5201
• RRID: AB_2283690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Jundishapur J Microbiol; Analysis of Subcellular Prefoldin 1 Redistribution During Rabies Virus Infection.; Authors - Jinyang Zhang