PFDN4 Polyclonal antibody

PFDN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16045-1-AP

Synonyms

C1, PFD4, PFDN4, prefoldin 4, prefoldin subunit 4, Protein C 1

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Validation Data Gallery

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  WB analysis using 16045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16045-1-AP (PFDN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 16045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16045-1-AP (PFDN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16045-1-AP (PFDN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, Raji cells, THP-1 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16045-1-AP targets PFDN4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFDN4 fusion protein Ag8938
• Full Name: prefoldin subunit 4
• Calculated Molecular Weight: 134 aa, 15 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC010953
• Gene Symbol: PFDN4
• Gene ID (NCBI): 5203
• RRID: AB_2162573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human PFDN4, prefoldin 4, is a subunit of the heterohexameric chaperone protein and belongs to the prefoldin family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. Previous reports have shown that PFDN4 is upregulated in breast cancer cell lines and tumor tissues (PMID: 11381030; PMID: 9630229). PFDN4 expression may be a prognostic factor in colorectal cancer (PMID: 20552408).

Publications

Species	Application	Title
human	WB	Sci Adv; miR-26a regulates extracellular vesicle secretion from prostate cancer cells via targeting SHC4, PFDN4, and CHORDC1.; Authors - Fumihiko Urabe
mouse	WB,IF	Biomark Res; Single-cell and bulk RNA-seq unveils the immune infiltration landscape associated with cuproptosis in cerebral cavernous malformations; Authors - Chengwei Chen