PFDN5 Polyclonal antibody

PFDN5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 15078-1-AP

Synonyms

PFD5, MM1, c-Myc-binding protein Mm-1

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Validation Data Gallery

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  WB analysis of HepG2 using 15078-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15078-1-AP (PFDN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15078-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15078-1-AP (PFDN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15078-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15078-1-AP (PFDN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15078-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15078-1-AP (PFDN5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse pancreas tissue, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15078-1-AP targets PFDN5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFDN5 fusion protein Ag7150
• Full Name: prefoldin subunit 5
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC003373
• Gene Symbol: PFDN5
• Gene ID (NCBI): 5204
• RRID: AB_2162703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Brain Sci; Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest; Authors - Qiang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (08-14-2019)

The antibody works good in mouse brain tissue in western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain