Tested Applications
| Positive WB detected in | pig stomach tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33764-1-AP targets PGA4 in WB, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37827 Product name: Recombinant human PGA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-261 aa of NM_001079808.3 Sequence: ACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDQSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITM Predict reactive species |
| Full Name | pepsinogen 4, group I (pepsinogen A) |
| Calculated Molecular Weight | 42kDa，388aa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | NM_001079808.3 |
| Gene Symbol | PGA4 |
| Gene ID (NCBI) | 643847 |
| RRID | AB_3743072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P0DJD7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PGA4(Pepsinogen A4), also known as pepsinogen A, is a member of Peptidase A1 family. PGA4 is the precursor protein of digestive enzyme Pepsin, which is synthesized and secreted by the chief cells of gastric mucosa. Autocatalytic cleavage occurs under acidic conditions (pH 1-3), and about 41-44 amino acid fragments at the N-terminal are removed to form active pepsin. Serum pepsinogen level can be used as an important biomarker: screening index of atrophic gastritis, early diagnosis and screening of gastric cancer. The ratio (PGR) of pepsinogen I(PGI, mainly PGA) to pepsinogen II(PGII) is an important parameter to evaluate the function of gastric mucosa. The observed molecular weight is 42-45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGA4 antibody 33764-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
