PGC Polyclonal antibody

PGC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28532-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Pepsinogen II, EC:3.4.23.3, PGⅡ

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse stomach tissue, rat stomach tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

28532-1-AP targets PGC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGC fusion protein Ag29347 Predict reactive species
Full Name progastricsin (pepsinogen C)
Calculated Molecular Weight 388 aa, 42 kDa
Observed Molecular Weight34-42 kDa
GenBank Accession NumberBC073740
Gene Symbol PGC
Gene ID (NCBI) 5225
RRIDAB_2918173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20142
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PGC antibody 28532-1-APDownload protocol
IHC protocol for PGC antibody 28532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Biosci

Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy

Authors - Mei-Fei Wu
humanWB

Transgenic Res

The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells

Authors - Hua-Chuan Zheng
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