HPGDS Polyclonal antibody

HPGDS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22522-1-AP

Synonyms

PGDS, Glutathione-requiring prostaglandin D synthase, Glutathione-dependent PGD synthase, Glutathione S-transferase, Glutathione S transferase

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Validation Data Gallery

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  WB analysis using 22522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22522-1-AP (HPGDS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 22522-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22522-1-AP (HPGDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22522-1-AP (HPGDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22522-1-AP (HPGDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22522-1-AP (HPGDS antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22522-1-AP (HPGDS antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat spleen tissue, mouse small intestine tissue
• Positive IHC detected in: human placenta tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

22522-1-AP targets HPGDS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPGDS fusion protein Ag18290
• Full Name: prostaglandin D2 synthase, hematopoietic
• Calculated Molecular Weight: 199 aa, 23 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC020734
• Gene Symbol: HPGDS
• Gene ID (NCBI): 27306
• RRID: AB_2879115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hematopoietic prostaglandin D synthase (HPGDS) is expressed in human Th2 lymphocytes, antigen-presenting cells, mast cells and microglia. HPGDS converts PGH2 into PGD2, a mediator thought to play a pivotal role in airway allergy and inflammatory processes (PMID: 16624958). HPGDS deficiency is a critical factor that impedes cutaneous wound healing in diabetes. Overexpressing Hpgds in ADSCs could be an effective way to accelerate DW healing by alleviating inflammatory cells recruitment and increasing M2 polarization in the proliferation phase (PMID: 35922870). HPGDS is a cytosolic homodimer of 26 kDa subunits which reveal a well-defined active site, enabling a structure-based design of inhibitors (PMID: 24900177).

Publications

Species	Application	Title
mouse	IF	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
human	WB	EMBO Mol Med; Tranilast directly targets NLRP3 to treat inflammasome-driven diseases.; Authors - Yi Huang
mouse	WB	Molecules; Integrative Analysis of Proteomics and Metabolism Reveals the Potential Roles of Arachidonic Acid Metabolism in Hypoxia Response in Mouse Spleen; Authors - Yujing Guo