PGK1 Monoclonal antibody

PGK1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat







Cat no : 68035-1-Ig


MIG10, OK/SW cl.110, PGK1, PGKA, phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2

Tested Applications

Positive WB detected inA431 cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, Jurkat cells, human platelet tissue, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

68035-1-Ig targets PGK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGK1 fusion protein Ag12099
Full Name phosphoglycerate kinase 1
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC103752
Gene symbol PGK1
Gene ID (NCBI) 5230
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).


Product Specific Protocols
WB protocol for PGK1 antibody 68035-1-IgDownload protocol
IHC protocol for PGK1 antibody 68035-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols