PGK1 Monoclonal antibody
PGK1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B2F3
Cat no : 68035-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, Jurkat cells, human platelet tissue, rat testis tissue, mouse testis tissue |
Positive IHC detected in | human liver cancer tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Product Information
68035-1-Ig targets PGK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PGK1 fusion protein Ag12099 |
Full Name | phosphoglycerate kinase 1 |
Calculated molecular weight | 417 aa, 45 kDa |
Observed molecular weight | 40-45 kDa |
GenBank accession number | BC103752 |
Gene symbol | PGK1 |
Gene ID (NCBI) | 5230 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).
Protocols
Product Specific Protocols | |
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WB protocol for PGK1 antibody 68035-1-Ig | Download protocol |
IHC protocol for PGK1 antibody 68035-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |