Tested Applications
| Positive WB detected in | A431 cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, Jurkat cells, human platelet tissue, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68035-1-Ig targets PGK1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12099 Product name: Recombinant human PGK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-417 aa of BC103752 Sequence: MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI Predict reactive species |
| Full Name | phosphoglycerate kinase 1 |
| Calculated Molecular Weight | 417 aa, 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC103752 |
| Gene Symbol | PGK1 |
| Gene ID (NCBI) | 5230 |
| RRID | AB_2918777 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PGK1 antibody 68035-1-Ig | Download protocol |
| IF protocol for PGK1 antibody 68035-1-Ig | Download protocol |
| IHC protocol for PGK1 antibody 68035-1-Ig | Download protocol |
| WB protocol for PGK1 antibody 68035-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PGK1 Monoclonal antibody (catalog 68035-1-Ig) has a total of 10 citations appearing in these journals: Nat Commun, Cell Rep Med, Cell Death Dis, Cancer Lett, Transl Oncol, J Biol Chem, Mol Cell Probes, J Extracell Vesicles, Oncol Res, and Cancers (Basel). The research fields span cancer biology (pancreatic cancer, glioblastoma, leukemia, breast cancer, renal cell carcinoma, endometrial cancer), tumor metabolism and glycolysis, diabetic kidney disease, myocardial ischemia-reperfusion injury, and hypoxia-mediated muscle growth.
| Species | Application | Title |
|---|---|---|
Nat Commun Metabolic characterization of the tumor microenvironment orchestrates therapeutic strategies and clinical outcomes in pancreatic cancer. | ||
Cell Rep Med Phosphoglycerate kinase 1 contributes to diabetic kidney disease through enzyme-dependent and independent manners.
| ||
Cell Death Dis Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression. | ||
Cancer Lett PGK1/PHGDH axis drives radioresistance in glioblastoma stem cells.
| ||
Transl Oncol Tetraarsenic tetrasulfide triggers ROS-induced apoptosis and ferroptosis in B-cell acute lymphoblastic leukaemia by targeting HK2 | ||
J Biol Chem Hypoxia-inducible factor-1 alpha modulates muscle growth and the molting process through its regulation of glycolysis in Neocaridina davidi |
