PGK1 Monoclonal antibody

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  WB analysis using 68035-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68035-1-Ig (PGK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 68035-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PGK1 antibody (68035-1-Ig, Clone: 1B2F3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  IF Staining of HepG2 using 68035-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGK1 antibody (68035-1-Ig, Clone: 1B2F3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

PGK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68035-1-Ig

Clone No.1B2F3

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PRP 2, PGKA, OK/SW-cl.110, EC:2.7.2.3, Cell migration-inducing gene 10 protein

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Tested Applications

• Positive WB detected in: A431 cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, Jurkat cells, human platelet tissue, rat testis tissue, mouse testis tissue
• Positive IHC detected in: human liver cancer tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68035-1-Ig targets PGK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PGK1 fusion protein Ag12099
• Full Name: phosphoglycerate kinase 1
• Calculated Molecular Weight: 417 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC103752
• Gene Symbol: PGK1
• Gene ID (NCBI): 5230
• RRID: AB_2918777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P00558
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).

Publications

Species	Application	Title
human	WB	Transl Oncol; Tetraarsenic tetrasulfide triggers ROS-induced apoptosis and ferroptosis in B-cell acute lymphoblastic leukaemia by targeting HK2; Authors - Wenke Bai