PGLYRP2 Polyclonal antibody

PGLYRP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 23029-1-AP

Synonyms

HMFT0141, PGLYRP2, PGLYRPL, PGRP L, PGRPL, tagL, tagL alpha, tagl beta, TAGL like



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23029-1-AP is PGLYRP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGLYRP2 fusion protein Ag19336
Full Name peptidoglycan recognition protein 2
Calculated molecular weight 576 aa, 62 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC136551
Gene symbol PGLYRP2
Gene ID (NCBI) 114770
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peptidoglycan recognition protein 2 (PGLYRP2) is an N-acetylmuramoyl-L-alanine amidase that hydrolyzes peptidoglycan, a ubiquitous component of bacterial cell walls (PMID: 14506276). It is involved in innate immunity. PGLYRP2 is primarily expressed in liver and secreted into blood (PMID: 11461926). The apparent molecular mass measured by SDS-PAGE is lager than calculated mass of 62 kDa, which is likely due to N-glycosylation (PMID: 16054449).

Protocols

Product Specific Protocols
WB protocol for PGLYRP2 antibody 23029-1-APDownload protocol
IHC protocol for PGLYRP2 antibody 23029-1-APDownload protocol
IP protocol for PGLYRP2 antibody 23029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols