PGM3 Polyclonal antibody

PGM3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29686-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Synonyms

AGM1, DKFZp434B187, PAGM, PGM 3, PGM3, phosphoglucomutase 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 29686-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29686-1-AP (PGM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate hyperplasia using 29686-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human prostate hyperplasia using 29686-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 40x lens).

Positive WB detected in	A549 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected in	human prostate hyperplasia tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

29686-1-AP targets PGM3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag30504 Product name: Recombinant human PGM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 380-542 aa of BC001258 Sequence: STAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF Predict reactive species
Full Name	phosphoglucomutase 3
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC001258
Gene Symbol	PGM3
Gene ID (NCBI)	5238
RRID	AB_2918339
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95394
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for PGM3 antibody 29686-1-AP	Download protocol
WB protocol for PGM3 antibody 29686-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Cell Death Dis PSMD11 stabilizes PGM3 by antagonizing Parkin to promote bladder cancer progression through energy metabolism reprogramming. Authors - Yu Cheng KD Validated View Article
mouse	WB,IHC	Biomaterials Gut-confined fullerene reprograms lactate metabolism to Rescue hypoxia-induced exercise intolerance. Authors - Shiliang Chen View Article