PGM3 Polyclonal antibody

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• PGM3 Polyclonal antibody (29686-1-AP)

PGM3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29686-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Synonyms

AGM1, DKFZp434B187, PAGM, PGM 3, PGM3, phosphoglucomutase 3

• 

  WB analysis using 29686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29686-1-AP (PGM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate hyperplasia using 29686-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate hyperplasia using 29686-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29686-1-AP targets PGM3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30504

  Product name: Recombinant human PGM3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 380-542 aa of BC001258

  Sequence: STAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF

• Full Name: phosphoglucomutase 3
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC001258
• Gene Symbol: PGM3
• Gene ID (NCBI): 5238
• RRID: AB_2918339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95394
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PGM3 Polyclonal antibody (Cat# 29686-1-AP) has 2 total citations. These appear in Biomaterials and Cell Death & Disease. The research fields covered include metabolic reprogramming (lactate metabolism in gut tissue and energy metabolism in bladder cancer), exercise physiology, and oncology. Applications cited are WB and IHC, with species spanning human and mouse.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	Biomaterials; Gut-confined fullerene reprograms lactate metabolism to Rescue hypoxia-induced exercise intolerance.; Authors - Shiliang Chen
human	WB	Cell Death Dis; PSMD11 stabilizes PGM3 by antagonizing Parkin to promote bladder cancer progression through energy metabolism reprogramming.; Authors - Yu Cheng ; KD Validated