PGM3 Polyclonal antibody

PGM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29686-1-AP

Synonyms

AGM1, DKFZp434B187, PAGM, PGM 3, PGM3, phosphoglucomutase 3

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Validation Data Gallery

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  WB analysis using 29686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29686-1-AP (PGM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 29686-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 29686-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29686-1-AP targets PGM3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGM3 fusion protein Ag30504
• Full Name: phosphoglucomutase 3
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC001258
• Gene Symbol: PGM3
• Gene ID (NCBI): 5238
• RRID: AB_2918339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.