Tested Applications
| Positive WB detected in | C2C12 cells, HeLa cells, HepG2 cells, HuH-7 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17149-1-AP targets PGS1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10961 Product name: Recombinant human PGS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-549 aa of BC025951 Sequence: LIPERFNETIGLQHIKVYLFDNSVILSGANLSDSYFTNRQDRYVFLQDCAEIADFFTELVDAVGDVSLQLQGDDTVQVVDGMVHPYKGDRAEYCKAANKRVMDVINSARTRQQMLHAQTFHSNSLLTQEDAAAAGDRRPAPDTWIYPLIQMKPFEIQIDEIVTETLLTEAERGAKVYLTTGYFNLTQAYMDLVLGTRAEYQILLASPEVNGFFGAKGVAGAIPAAYVHIERQFFSEVCSLGQQERVQLQEYWRRGWTFHAKGLWLYLAGSSLPCLTLIGSPNFGYRSVHRDLEAQIAIVTENQALQQQLHQEQEQLYLRSGVVSSATFEQPSRQVKLWVKMVTPLIKNFF Predict reactive species |
| Full Name | phosphatidylglycerophosphate synthase 1 |
| Calculated Molecular Weight | 556 aa, 63 kDa |
| Observed Molecular Weight | 63~70 kDa |
| GenBank Accession Number | BC025951 |
| Gene Symbol | PGS1 |
| Gene ID (NCBI) | 9489 |
| RRID | AB_2252418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q32NB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PGS1 (Phosphatidylglycerophosphate Synthase 1) catalyze the synthesis of phosphatidylglycerophosphate, a critical and rate-limiting step in the production of cardiolipin and other anionic phospholipid. PGS1 acts as a scaffold that forms the core of a larger cardiolipin synthesis complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGS1 antibody 17149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
FASEB J Loss of STARD7 Impairs Mitochondrial Phospholipid Homeostasis and Contributes to Mitochondrial Myopathy. | ||
Autophagy PRKAB2 as a tumor suppressor in renal cell carcinoma: inhibiting mitophagy via the LRPPRC-PRKN/parkin interaction and cardiolipin biosynthesis. |
