Tested Applications
| Positive WB detected in | C2C12 cells, HeLa cells, HepG2 cells, HuH-7 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17149-1-AP targets PGS1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10961 Product name: Recombinant human PGS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-549 aa of BC025951 Sequence: LIPERFNETIGLQHIKVYLFDNSVILSGANLSDSYFTNRQDRYVFLQDCAEIADFFTELVDAVGDVSLQLQGDDTVQVVDGMVHPYKGDRAEYCKAANKRVMDVINSARTRQQMLHAQTFHSNSLLTQEDAAAAGDRRPAPDTWIYPLIQMKPFEIQIDEIVTETLLTEAERGAKVYLTTGYFNLTQAYMDLVLGTRAEYQILLASPEVNGFFGAKGVAGAIPAAYVHIERQFFSEVCSLGQQERVQLQEYWRRGWTFHAKGLWLYLAGSSLPCLTLIGSPNFGYRSVHRDLEAQIAIVTENQALQQQLHQEQEQLYLRSGVVSSATFEQPSRQVKLWVKMVTPLIKNFF Predict reactive species |
| Full Name | phosphatidylglycerophosphate synthase 1 |
| Calculated Molecular Weight | 556 aa, 63 kDa |
| Observed Molecular Weight | 63~70 kDa |
| GenBank Accession Number | BC025951 |
| Gene Symbol | PGS1 |
| Gene ID (NCBI) | 9489 |
| RRID | AB_2252418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q32NB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PGS1 (Phosphatidylglycerophosphate Synthase 1) catalyze the synthesis of phosphatidylglycerophosphate, a critical and rate-limiting step in the production of cardiolipin and other anionic phospholipid. PGS1 acts as a scaffold that forms the core of a larger cardiolipin synthesis complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGS1 antibody 17149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PGS1 Polyclonal Antibody (Cat# 17149-1-AP) has 4 citations published in Autophagy, Sci China Life Sci, FASEB J, and Neurobiol Dis. The associated research fields include renal cell carcinoma and mitophagy, cardiac hypertrophy via autophagy, mitochondrial phospholipid homeostasis and myopathy, and phosphatidylglycerol metabolism in pain hypersensitivity (Fabry disease model). All cited applications are Western blot in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Autophagy PRKAB2 as a tumor suppressor in renal cell carcinoma: inhibiting mitophagy via the LRPPRC-PRKN/parkin interaction and cardiolipin biosynthesis. | ||
FASEB J Loss of STARD7 Impairs Mitochondrial Phospholipid Homeostasis and Contributes to Mitochondrial Myopathy. | ||
Neurobiol Dis Dysregulated phosphatidylglycerol metabolism in dorsal root ganglia contributes to pain hypersensitivity in a mouse model of Fabry disease. |
