PHF12 Polyclonal antibody

PHF12 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24485-1-AP

Synonyms

KIAA1523, PF1, PHD factor 1, PHD finger protein 12, PHF12

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Validation Data Gallery

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  WB analysis of mouse brain using 24485-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 24485-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24485-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 24485-1-AP

  IP result of anti-PHF12 (IP:24485-1-AP, 4ug; Detection:24485-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse lung tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 24485-1-AP is PHF12 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF12 fusion protein Ag18471
• Full Name: PHD finger protein 12
• Calculated Molecular Weight: 1004 aa, 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC121043
• Gene Symbol: PHF12
• Gene ID (NCBI): 57649
• RRID: AB_2879566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plant homeodomain [PHD] zinc finger protein 12 (PHF12), also known Pf1, is a member of the PHD zinc finger family of proteins. Pf1 associates with a chromatin-interacting protein complex comprised of MRG15, Sin3B, and histone deacetylase 1 (HDAC1) that functions as a transcriptional modulator.

Publications

Species	Application	Title
human	WB	EMBO Rep; MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.; Authors - Gongwei Wu
human	WB	J Transl Med; PHF12 regulates HDAC1 to promote tumorigenesis via EGFR/AKT signaling pathway in non-small cell lung cancer; Authors - Yiru Kong ; KD Validated